Clinical Lab Handbook

Radiometer ABL uses a potentiometric method, amperometric method, and an optical measurement system.

1. Potentiometry: The potential of a sensor chain is recorded using a voltmeter, and related to the concentration of the sample (the Nernst equation). The potentiometric measuring principle is applied in the pH, pCO2, K+, Na+, Ca2+ and Cl— sensors.
2. Amperometry: The magnitude of an electrical current flowing through a sensor chain is proportional to the concentration of the substance being oxidized or reduced at an electrode in the chain. The Amperometric measuring principle is applied in the cGlu and cLac sensors.
3. Optical pO2: The optical system for pO2 is based on the ability of O2 to reduce the intensity and time constant of the phosphorescence from a phosphorescent dye that is in contact with the sample. This measuring principle is applied in the pO2 sensor.
4. Spectrophotometry: Light passes through a cuvette containing a hemolyzed blood sample. The specific wavelengths absorbed and their intensity generates an absorption spectrum used to calculate oximetry parameters. This measuring principle is used for measuring ctHb, sO2, FO2Hb, FCOHb, FHHb, FMetHb, FHbF and ctBil.

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