Clinical Lab Handbook

Container/Tube: SST

Alternate Container: Lithium Heparin, Sodium Heparin, Red Top

Specimen Volume: 5 mL of serum

Collection Instructions: Fasting (10-12 hour). Allow specimen to clot for 30 minutes. Spin specimen in centrifuge.

Centrifuge specimens and remove the serum or plasma from the cellular material within 4 hours of collection.

Enzymatic Colorimetric

The TRIG test is a multilayered, analytical element coated on a polyester support. The analysis is based on an enzymatic method as described by Spayd et al.

A drop of patient sample is deposited on the slide and is evenly distributed by the spreading layer to the underlying layers. The Triton X-100 surfactant in the spreading layer aids in dissociating the triglycerides from lipoprotein complexes present in the sample. The triglyceride molecules are then hydrolyzed by lipase to yield glycerol and fatty acids. Glycerol diffuses to the reagent layer, where it is phosphorylated by glycerol kinase in the presence of adenosine triphosphate (ATP). In the presence of L-α-glycerophosphate oxidase, L-α-glycerophosphate is then oxidized to dihydroxyacetone phosphate and hydrogen peroxide. The final reaction involves the oxidation of a leuco dye by hydrogen peroxide, catalyzed by peroxidase, to produce a dye.

The density of the dye formed is proportional to the triglyceride concentration present in the sample and is measured by reflectance spectrophotometry.

Triglyceride measurements are used in the diagnosis and treatment of patients with diabetes mellitus, nephrosis, liver obstruction, other diseases involving lipid metabolism, or various endocrine disorders.

Normal: <150 mg/dL

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