Clinical Lab Handbook

Container/Tube: SST

Alternate Specimen: Plasma specimens w/ EDTA, CPD, CPDA-1, heparin or sodium citrate as anticoagulants/Red Top

Specimen Volume: 2.5 mL of serum

Collection Instructions: Allow specimen to clot for 30 minutes. Spin specimen in centrifuge.

Specimen Viability:

Macroscopic Non-Treponemal Flocculation

Treponema pallidum, the etiological agent of syphilis, induces the production of at least two types of antibodies in human infection: anti-treponemal antibodies that can be detected by FTA-ABS antigen, and anti-nontreponemal antibodies (reagin) that can be detected by RPR antigen.

The Sure-Vue® RPR (rapid plasma regain) for Syphilis is a qualitative nontreponemal flocculation test for the detection of reagin antibodies in human serum and plasma as a screening test in syphilis serology. Treponema pallidum, the etiological cause of syphilis induces the production of reagin antibodies if present.

The Sure-Vue® RPR is an 8 minute macroscopic nontreponemal flocculation test to be used for the detection of reagin. The micro-particulate carbon RPR antigen enhances the visual discrimination between reactive and nonreactive results. The reagin-type antibody binds with the antigen that is composed of a complex of cardiolipin, lecithin and cholesterol particles with activated charcoal; the result of this antigen-antibody reaction is macroscopic agglutination.

Non reactive

Monday through Friday

86592

Note: All REACTIVE samples will be sent to MDCH (MI State Lab) for confirmatory testing.