Clinical Lab Handbook

Must be an inpatient at MyMichigan Health - Midland. Specimen must arrive unspun to the lab within 15 minutes of draw.

Specimen: Blue top (sodium citrate)

Specimen Viability: 2hrs, Room Temperature

Collection

1. Collect specimens using standard laboratory procedures.
2. Use a 21 gauge or larger needle, preferably a butterfly needle with 12 inch tubing.
3. Discard the first 3ml of blood by drawing a clear waste tube
4. Do not use waste tubes that contain clot activators
5. Gently invert the tube 5 times to mix
6. Place the tube horizontally on a flat surface or transport horizontally until time to analyze sample
7. Specimens may be transported at room temperature via the pneumatic tube system when packaged in a protective and enclosed foam carrier
8. Alternatively, specimens may be hand delivered via a courier to the laboratory

The TEG analyzer approach to the monitoring of patient hemostasis is based on the following:

1. The end result of the hemostasis process is the clot.
2. The clot's physical and developmental properties (rate, strength, and stability) affect whether the patient will have normal hemostasis, will hemorrhage or will develop thrombosis.

Disposable assay cartridges contain all of the components necessary to allow the analyzer to prepare samples and perform hemostasis tests. The analyzer automatically draws the blood into the active area of the cartridge, meters the exact amount required for the test, and mixes it with the reagents spotted in the cartridge. The analyzer then monitors the harmonic motion of a pendant drop of blood in response to external vibration. As the sample transitions from a liquid state to a gel-like state during clotting, the modulus of elasticity and resonant frequency increase. The analyzer measures these variations in resonant frequency during clotting and lysis. The results are displayed in a table and on a graphical tracing that reflects a hemostasis profile of clot formation.

The resulting hemostasis profile is a measure of the time it takes for the first measurable clot to be formed, the kinetics of clot formation, the strength of the clot, and the breakdown of the clot, or fibrinolysis.

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