Clinical Lab Handbook

Urine-No Preservative

VITROS Chemistry Products AMPH Reagent is used on the VITROS Integrated Systems for the qualitative determination of amphetamines (AMPH) in human urine using a cutoff of either 500 ng/mL. Measurements obtained with the VITROS AMPH method are used in the diagnosis and treatment of amphetamines use or overdose.

The VITROS Chemistry Products AMPH assay provides only a preliminary test result. A more specific alternative chemical method must be used to confirm a result obtained with this assay. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug-of-abuse test result, particularly when evaluating a preliminary positive result.

“Amphetamines” refers to a group of drugs that includes d-amphetamine, d-methamphetamine, d,l-amphetamine, Methylenedioxyamphetamine (MDA), and methylenedioxymethamphetamine (MDMA). Amphetamines suppress appetite and produce central nervous system and cardiovascular stimulation. Amphetamines can be taken orally, intravenously, or by inhalation.

Amphetamines are readily absorbed from the gastrointestinal tract and are then either deactivated by the liver or excreted unchanged in the urine. Amphetamine is metabolized to deaminated acids and hydroxylated metabolites. Approximately 70% of a dose is excreted within 24 hours. Of the amount excreted, about 30% is unchanged drug. Methamphetamine is partially metabolized to amphetamine, its major active metabolite. Approximately 43% of a dose is excreted unchanged within 24 hours. The rate of excretion and the pathway used depends on urinary pH. Acidic conditions will increase the amount and rate of excretion of unchanged drug and alkaline conditions will delay excretion and increase the amount metabolized.

The VITROS AMPH assay detects both d-amphetamine and d-methamphetamine. The assay also detects l‑amphetamine, l‑methamphetamine, d,l-amphetamine, d,l-methamphetamine, methylenedioxyamphetamine (MDA), and, to a lesser extent, methylenedioxymethamphetamine (MDMA) and methylenedioxyethylamine (MDEA) in human urine. Measurements of amphetamines in urine are used in the diagnosis and treatment of amphetamine use and overdose.

80307

Qualitative, Negative/Positive

Cutoff 500 ng/ml

Instructions for Use: Amphetamine.pdf (Qualitative Result Mode)

Refrigerated, 7 days